mouse il 10 elisa kit  (R&D Systems)

Journal: Non-coding RNA Research

Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma

doi: 10.1016/j.ncrna.2026.03.003

Figure Lengend Snippet: circSMAD4 drives tumor-educated M2-like polarization of macrophages and promotes tumor-cell aggressiveness. (A) Workflow for generating TC-hMDMs and TC-BMDMs, circSMAD4 knockdown, and downstream functional assays. (B) RT–qPCR analysis of M1-associated markers (MHC-II [HLA-DRA in TC-hMDMs; H2-Ab1 in TC-BMDMs], NOS2, and CD86) and M2-associated markers (CD163, CD206, and ARG1) in TC-hMDMs and TC-BMDMs. (C) Representative flow-cytometry histograms for HLA-DR, iNOS, CD86, CD163, CD206, and ARG1 in TC-hMDMs. Gating strategy and marker thresholds were defined based on FMO controls (see ). (D) Flow-cytometry quantification of marker-positive cells in TC-hMDMs and TC-BMDMs. (E) ELISA of IL-10, TGF-β, and iNOS in culture supernatants. (F) CCK-8 assays of A549 and LLC cells. (G) Colony-formation assays of A549 and LLC cells with quantification. (H) Bioluminescence-based growth readouts of patient-derived LUAD organoids (PDO #1 and PDO #2) after co-culture with TC-hMDMs. (I) Immunoblot analysis of EMT-related proteins (E-cadherin, N-cadherin, Vimentin) in A549 and LLC cells. (J) Transwell migration and invasion assays of A549 and LLC cells with quantification. Scale bar, 50 μm. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001; ns, not significant.

Article Snippet: For mouse experiments, mouse IL-10 was measured using the Mouse IL-10 ELISA Kit (R&D Systems, Cat# M1000B), and mouse TGF-β1 was measured using the Mouse TGF beta-1 ELISA Kit (Invitrogen, Cat# BMS608-4), following the manufacturers’ instructions.

Techniques: Knockdown, Functional Assay, Quantitative RT-PCR, Flow Cytometry, Marker, Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Derivative Assay, Co-Culture Assay, Western Blot, Migration

Journal: Redox Biology

Article Title: Hepatic supersulfides attenuate acetaminophen-induced liver injury via enhanced detoxification and anti-inflammatory mechanisms

doi: 10.1016/j.redox.2026.104140

Figure Lengend Snippet: Suppression of proinflammatory cytokine production and M1-type macrophage polarization by a supersulfide donor in livers from APAP-treated mice. (A–C) Levels of IL-1β, IL-6, and IL-10 in liver tissue. Mice were intraperitoneally administered APAP (330 mg/kg), followed by subcutaneous NAC-S2 administration at 30 min and 2 h post-APAP. Livers were harvested 24 h after APAP administration, and cytokine levels were quantified by ELISA. (D) Western blot analysis of hepatic iNOS and Arg-1 expression. (E, F) Quantification of Western blot band intensities. Relative protein levels were normalized to β-actin. Uncropped blots are shown in . Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc multiple comparisons test. Data were expressed as means ± SD (n ≥ 3). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001; # P < 0.05, # # P < 0.01, # # # P < 0.001 compared to control group.

Article Snippet: Mouse IL-1β/IL-1F2 Quantikine ELISA Kit (MLB00C), Mouse IL-6 Quantikine ELISA Kit (M6000B-1), and Mouse IL-10 Quantikine ELISA Kit (M1000B) were obtained from R&D Systems (Minneapolis, MB, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Control

Journal: Redox Biology

Article Title: Hepatic supersulfides attenuate acetaminophen-induced liver injury via enhanced detoxification and anti-inflammatory mechanisms

doi: 10.1016/j.redox.2026.104140

Figure Lengend Snippet: Effects of NAC-S2, oxNAC, and NAC on APAP-induced liver injury. (A) Experimental protocol. Mice were intraperitoneally administered APAP (330 mg/kg), followed by subcutaneous administration of sulfur compounds (NAC-S2, oxNAC, or NAC) at 30 min and 2 h post-APAP. Blood was collected at 8, 12, and 24 h post-APAP, and liver tissue was harvested at 24 h post-APAP. (B, C) Time-course of serum ALT and AST levels. (D) Quantification of necrotic area in liver. Macroscopic appearance and H&E staining of liver tissues are shown in Hepatic cytokine levels: IL-1β (E), IL-6 (F), and IL-10 (G). (H) Western blot analysis of iNOS and Arg-1 expression in liver. (I, J) Quantification of Western blot band intensities. Relative protein levels were normalized to β-actin. Uncropped blots are shown in . Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc multiple comparisons test. Data were expressed as means ± SD (n ≥ 3). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001; # P < 0.05, # # P < 0.01, # # # P < 0.001 compared to control group.

Article Snippet: Mouse IL-1β/IL-1F2 Quantikine ELISA Kit (MLB00C), Mouse IL-6 Quantikine ELISA Kit (M6000B-1), and Mouse IL-10 Quantikine ELISA Kit (M1000B) were obtained from R&D Systems (Minneapolis, MB, USA).

Techniques: Staining, Western Blot, Expressing, Control

Journal: Redox Biology

Article Title: Hepatic supersulfides attenuate acetaminophen-induced liver injury via enhanced detoxification and anti-inflammatory mechanisms

doi: 10.1016/j.redox.2026.104140

Figure Lengend Snippet: Effects of the supersulfide donor TGS4 on APAP-induced liver injury. (A) Experimental protocol. Mice were intraperitoneally administered APAP (330 mg/kg), followed by subcutaneous injection of TGS4 at 30 min and 2 h post-APAP. Two different doses were used as indicated. Blood was collected at 8, 12, and 24 h post-APAP, and liver tissue was harvested at 24 h post-APAP. (B, C) Time-course of serum ALT and AST levels. (D–F) Hepatic cytokine levels: IL-1β (D), IL-6 (E), and IL-10 (F). Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc multiple comparisons test. Data were expressed as means ± SD (n ≥ 4). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001; # P < 0.05, # # P < 0.01, # # # P < 0.001 compared to control group.

Article Snippet: Mouse IL-1β/IL-1F2 Quantikine ELISA Kit (MLB00C), Mouse IL-6 Quantikine ELISA Kit (M6000B-1), and Mouse IL-10 Quantikine ELISA Kit (M1000B) were obtained from R&D Systems (Minneapolis, MB, USA).

Techniques: Injection, Control